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The 32 Cuban officers were part of Maduro’s security detail killed in Caracas to seize the former leader and bring him to the U.S. to face drug trafficking charges.

“Humanity is experiencing something very complex, and (the US) is governed by a president who considers himself an emperor,� said René González, 64, one of the protesters.

“We must show him that ideas are worth more than weapons," he said. "This march is a message of our unity. Independence is sacred, and we will defend it tooth and nail if necessary.�

Cuba’s national hymn rang out at Friday’s demonstration as large Cuban flags waved in the chilly wind and big waves broke nearby along Havana’s famed pier. President Miguel Díaz-Canel shook hands with members of the crowd clad in jackets and scarves before speaking to them.

“The current U.S. administration has opened the door to an era of barbarism, plunder and neo-fascism,� he said.

The demonstration was a show of popular strength after U.S. President Donald Trump recently demanded that Cuba before it is “too late.� He did not explain what kind of deal.

Trump also has said that Cuba will no longer live off Venezuela’s oil and money. Experts say the move could have catastrophic consequences since Cuba is already struggling with severe blackouts.

“No one here surrenders," Díaz-Canel said. “The current emperor of the White House and his infamous secretary of state haven’t stopped threatening me.�

Washington has maintained a policy of sanctions against Cuba since the 1960s to pressure the island's government to improve its human rights record, end its one-party communist system and allow democracy. The sanctions have been further tightened during Trump’s presidency, suffocating the island’s economy.

“Cuba does not have to make any political concessions, and that will never be on the table for negotiations aimed at reaching an understanding between Cuba and the United States,� Díaz-Canel said. “It is important that they understand this. We will always be open to dialogue and improving relations between our two countries, but only on equal terms and based on mutual respect.�

After the president's speech, the demonstration transitioned into a parade that Cubans call a “combatant march,� a custom that originated during the time of the late leader Fidel Castro. The crowd was led by a line of people holding pictures of the 32 officers killed.

“Down with imperialism!� the crowd yelled. “Cuba will prevail!"

The demonstration was organized a day after tens of thousands of Cubans gathered at the headquarters of the Ministry of the Armed Forces to the 32 officers slain.

Their remains arrived home on Thursday morning, and they are scheduled to be laid to rest on Friday afternoon in various cemeteries following memorial ceremonies in all of Cuba’s provincial capitals.

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Associated Press reporter Dánica Coto in San Juan, Puerto Rico contributed to this report.